In all but one non-dipteran insect species, no intervening genes were identified in the RD gene cluster, the only exception being in B. mori where five annotated genes fall between BmCG42267 and BmCG34145.
This QTL region harbours more than six hundred annotated genes.
Fifty annotated genes were identified (see Table 3).
If, during the course of the sequencing or based on new experimental evidence, a new gene is detected between the two already annotated genes, the new gene will be assigned a number between the two previously annotated genes, using the tenth number space.
Among these, Blast2GO includes a tool for performing enrichment analysis, i.e., the identification of GO annotations whose abundance is significantly different between two sets of annotated genes.
Block 3 492 kb long (between H3GA0020849 and ASGA0032595) contained four annotated genes, namely, t-complex 11, testis-specific (TCP11); signal peptide, CUB domain, EGF-like 3 (SCUE3); peroxisome proliferator-activated receptor delta (PPARD); and FK506 binding protein 5 (FKBP5).
There were ninety-four ACC OXIDASE annotated genes, nine of which have been published [ 8].
Between the matched annotated genes in the two libraries, 7,349 (83%) genes were present in both libraries.
Among these sixteen genes, ten genes were down-regulated including four well- annotated genes, MSRA, WHSC1L1, ARHGEF10 and NAT1.
