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In our analyses, there was extensive congruence between trees based on N- and C-termini of silk gene family members (Figure 4C).
In order to analyze the congruence among the gene trees above, we firstly measured topological similarity between trees based on the Robinson-Foulds distance.
Noncongruence between trees based on the sequences of single DNA fragments indicated that recombination events had occurred (data not shown) [23].
To assess the extent of congruence between trees based on concatenated alignments and individual phylogenies, we compare the topology of phylogenies of genes encoded in the yeast genome with fungal species trees reconstructed from the concatenated alignments of widespread proteins present across different sets of fungal species.
Closer here means a smaller distance between trees based on the Rooted Branch Score.
Addressing some of the incongruence between trees based on individual genes (18S, 28S, 16S, CO1) and those based on complete mitochondrial genomes in euthyneuran phylogenetics is a long-term goal that is beyond the scope of the present study.
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In all three trees chosen by different statistical methods, distance between tree based on whole genome alignment and SNP concatemers derived from CS was always smaller than that based on whole genome alignment and SNP concatemers derived from H37Rv (Table 3).
There are two contradictions between the trees based on RAG-1 and myoglobin intron II, respectively (Fig. 9).
Interestingly, no statistical incongruence between all trees based on the GHF5 catalytic domain and on the CBM2 was found for the whole and the PPN dataset (Table 1).
By far the most common method of detecting events involving eukaryotes is to observe incongruence between phylogenetic trees based on a gene and the tree that is considered (on the basis of other evidence) to reflect the evolution of the organism in which the gene is encoded.
Topological differences between the trees based on the partitioned and combined data do not present a problem for choosing among alternative models of sequence evolution, because tree topology does not strongly affect model estimation unless long internal branches are involved [ 90].
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