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A positive correlation (R2 = 0.8) between transgene expression level and α-copaene abundance was observed.
In the LV-GFP control group, there was no correlation between transgene expression level and ERG amplitudes.
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For transgenic plants of the three 35S:: HvMLO T2 families, positive correlation was found between average D.I. and transgene expression level of corresponding T1 plants (Fig. 3 and Additional file 2 and 3).
The correlation between the transgene copy number and transgene expression level was assessed by analysing the testis of viable descendants.
However, hydrogels with HA maintained this transgene expression level for an additional 2 weeks before declining.
pIRES2-ZsGreen1-hBMP2 dual expression plasmid containing both ZsGreen1 GFP reporter gene and BMP2 functional gene was fabricated to monitor the transgene expression level.
pIRES2-ZsGreen1-hBMP2 dual expression plasmid containing both the ZsGreen1 GFP reporter gene and the BMP2 functional gene was constructed for monitoring the transgene expression level.
These data indicate that the transgene expression level decreases with aging in vivo.
Screen gene copy numbers and transgene expression level regularly.
The two most important criteria for evaluating a regulation system are the regulation factor (the ratio of maximum transgene expression level to minimum transgene expression level) and the maximum induced transgene expression level.
A housekeeping gene, UBC, was used as a reference to normalize the transgene expression level.
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