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NCBI's UniGene contains gene-oriented clusters of transcript sequences obtained by alignments between transcript sequences and genomic sequences originating from the same gene.
Finally, blastx alignment (evalue < 0.00001) between transcript sequences and protein databases such as NR, Swiss-Prot, KEGG and COG was performed, and the best aligning results were used to decide sequence direction of unigene sequences.
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While several other tools exist for generating spliced alignments between transcript sequences (ESTs and FL-cDNAs), including sim4 [ 87] and BLAT [ 88], they were not designed for aligning spliced transcripts of diverged species, but rather for accurately mapping near-identical transcript sequences.
Finally, analysis of allelic variations between transcript sequences of several Hevea clones could be useful for the development of functional molecular markers.
CT gene loci were identified in both human and chimpanzee based on sequence identity between the human transcript sequences and human or chimpanzee genomic sequences.
Volders, P.J. et al. LNCipedia: a database for annotated human lncRNA transcript sequences and structures.
To distinguish between valid transcript sequence and spuriously assembled sequence we compared the post-clustering set of contigs to multiple Dipteran insect transcriptomes, searched for open reading frames and compared translated protein sequences to functional protein domain databases as a means to identify contigs with bioinformatic associations with other species.
Comparison between the cDNA transcript sequence and corresponding genomic sequence revealed that all of the intron/exon boundaries of the five alternatively spliced transcripts are flanked by canonical AG/GT donor and acceptor splice sites [59].
Finding exact alignments between probe and transcript sequences was used to attribute signal intensities of probes to transcript (or gene) expression.
Comparison between genome and transcript sequences using common numerous specimens may resolve this uncertainty.
Matches including insertion/deletions between probe and transcript sequences can also give rise to strong cross-hybridization signals.
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