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Summary of principal component analysis of 10-dimensional vectors of correlation coefficients between transcript intensity and the 5'/3' beta actin ratio.
Another interesting feature of the distributions of correlation coefficients identified in this study is the significant predominance and strength of negative correlations between transcript intensity and post chip RNA quality.
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a Transcript intensity was determined and differentially expressed genes (DEG) with an uncorrected p < 0.05 and a change in intensity between Fe-sufficient and Fe-deficient treatments of 50% were selected for each of the five time points analyzed.
We will show that our approach consistently explains previous observations such as the effect of RNA quality on transcript intensity level [ 4] and correlations between probe intensity and probe position along the transcripts and their effect on expression measures [ 21].
The relationship between transcript abundance and intensity is affected by parasitic effects owing to the "technical" variability of repeated measurements and systematic biases which disturb the one-to-one relationship between the input and the output quantity of the measurement [ 1].
We calculated the median intensity of each transcript over the 3 YD arrays and the 3 SP arrays and took their average (the transcript intensity score).
This approach provides an empirically verified linear relationship between signal intensity and transcript level for each array.
For example it has been shown that the probes saturate at higher transcript concentrations which gives rise to a non-linear relation between intensity and transcript concentration.
Cell size and the transcript detection thresholds were filtered to accept cell sizes and transcript intensities that followed the Gaussian distribution.
In brief, DASL transcript intensities were quantile normalized in GenomeStudio and replicates were mean combined.
(a) Relation between transcript abundance and DNA accessibility.
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