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Furthermore, there was no significant difference between total and antigen-specific IgG4 responses in patient and control groups (not shown).
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Serum samples for total and antigen-specific (IgE) levels were collected using a population-based, nested, case control design.
Total and antigen-specific serum IgE detections were performed for staple foods and the putative allergens according to the patients' history or skin tests.
There was a strong correlation between serum IgG4 and antigen-specific responses.
We demonstrated that ETEN arise from the ERC upon cognate interaction between human DC and antigen-specific CD8+ T cells.
We also found that fewer total and Ova257-264 antigen-specific CD8+ T cells accumulated in p47phox−/− and gp91phox−/− spleens 7 days after infection than similarly treated WT spleens.
After 33 days of expansion, 17 72% of total cells in the cultures were antigen-specific CD8+ T cells (Figure 9A and B), and antigen-specific T cells had been expanded between 2.900 (CMV pp65) and 19.000 fold (MelanA).
It involves innate immunity and natural killer cells, and antigen-specific immunity directed against cancer-specific antigens and viral antigens.
PEM increased both nonspecific total IgA and oral antigen-specific IgA in serum without alteration of gut permeability.
In an aviary study we vaccinated females against a novel antigen that these birds would not otherwise encounter in their natural environment, and measured both antigen-specific and total antibody concentration in the plasma of females, yolks, and nestlings.
For non-stimulated controls, 25 μl of PBS was added to wells and for antigen-specific stimulation, a total of 25 μl of BPI3V antigen in PBS was added (final antigen concentration of 4 μg/ml).
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