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Means followed by the same letter state not significant differences between times of sampling within each treatment, as resulting by one-way ANOVA (P ≤ 0.05) and Tukey's HSD test (P ≤ 0.05).
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For every gene, we centered individually each of the separate profiles corresponding to a given time of day, thus eliminating potential circadian clock driven differences between these times of sampling.
Two-factor analysis of variance (ANOVA) revealed significant (P ≤ 0.001) differences between the times of sampling (48, 72, 96 and 168 hpi) in the observed means for the fungal tef1 α DNA quantity and between the two treatments (non-pathogenic and pathogenic inoculated samples), and their interactions (Additional file 1: Table S1).
We found that swab samples lost viability if more than three days (at 4°C) had elapsed between time of sample taking and receipt following confirmation of positivity.
We examined the association between time of sample collection and the urinary concentrations in a one-way mixed model.
Both N2O and CH4 fluxes differed significantly between times of day of sampling.
In both studies, negative correlations were seen between all variables and the time of sampling.
To assess differences in water quality and coral physiology in the laboratory experiment, two linear mixed effects models were used to test: (i) controls only (differences between illuminated and shaded controls, and between different times of sampling during the day), and (ii) shaded treatments only (differences between 0, 33, 67 and 100% algal water concentrations, and time of sampling).
In addition, N2O flux differed significantly between time of day of sampling.
The number of sheep samples and the time of sampling varied between the farms.
Lengths and weights at the time of sampling varied between all groups.
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