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Otherwise, no significant differences between timepoints were identified.
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Residual outlier timepoints were identified using FSL's motion outlier detection program and integrated as additional confound variables in the first-level General Linear Model (GLM) analysis.
Comparisons of fluorescence and MRI measures between left (ligated) and right (non-ligated) carotid arteries and between timepoints were analyzed by the Wilcoxon signed-rank test.
Different metabolites were identified at different timepoints, of which several were directly correlated to the progression into hypoxemia.
As each timepoint only had a single replicate in transcript profiles, cold-regulated genes were identified using limma with a sliding window of three sequential timepoints as replicates.
The diameters of individual lumbrical muscle fibres were measured, and no discernible differences were identified at early- or mid-late-symptomatic timepoints (supplementary material Fig. S2A).
Moreover, glycine, N-acetylglutamate and allantoin were identified with significantly increased concentration at five, four and three timepoints, respectively.
Only 7/116 were identified with affective symptoms alone at the 10-month timepoint.
Therefore, subpopulations of microglia/macrophages were identified by immunohistochemistry and compared to structures exhibiting endogenous fluorescence signals at a cellular level at different timepoints after SCI.
Cells specific for CMV were identified by tetramer staining, and the frequencies of tetramer-specific cells were compared between 12 and 24 months post-diagnosis using a random effects model that allowed for the incorporation of subjects for whom data for one or other timepoint was missing.
Each timepoint element can be identified by a non-negative integer value representing when the data was recorded.
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