padellus, mahalebellus, rorrellus, yanawaganus, and eurinellus, Thus, for our sequences these two restriction sites are not diagnostic between Y. malinellus and Y. cagnagellus + Y. padellus, but rather between Y. padellus and the other two taxa.
These results suggest the GC bias between the two restriction enzymes may explain the difference in the abundance of TEs between the two BES groups.
We also performed linkage analysis of HWC2 (Ichitani et al. 2001) and tagged this locus between the two restriction fragment length polymorphism (RFLP) markers on the long arm of chromosome 4.
Part of the observed variation in AFLP data is caused by indels (insertions or deletions) between the two restriction sites at a single locus, resulting in amplicons of different sizes.
The AFLP mutations that are primary due to point mutations within the restriction site, with a small fraction of in/dels that occurs between the two restriction enzyme sites and that should originate co-dominant AFLP loci.
Both of these values were significantly greater than the FI of the controls and remained so for the 4 days of measurement, although after the first day there was no significant difference between the two restriction groups: FI had decreased to 6.61±0.54 g/day and 7.70±0.37 g/day for low and high restriction groups, respectively (P>0.05).
This SBP minigene was designed between two restriction sites SnaB I and Bsp1407I which allows easy insertion into the gVII of the wild-type helper plasmid M13cp.
The mutagenized fragments were digested with BglII and NdeI and inserted between the same two restriction sites in pJV09, to produce pJV12-pJV52 (Supplementary file 3).
The STE5pr (−602 to −1 of STE5) amplified from the W303 genome, the LAC12 amplified from pKR1B-LAC4-1 (Sreekrishna anDicksonon, 1985), and the CYC1ter amplified from pGREG506 (Jansen et al., 2005) were inserted into pRS304 (Sikorski and Hieter, 1989) with SacI-SpeI-SalI-XhoI to make the transcription unit of the transporter (each fragment was sequentially inserted between two restriction sites).
To test their structural and functional similarity, hybrids were constructed between EcoRI and RsrI, two restriction endonucleases recognizing the same DNA sequence and sharing 50% amino acid sequence identity.
