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Having identified ERK1/2 as a putative target of both BF/S+L/Ep and cytopiloyne, we next aimed to distinguish the mechanistic difference between these two preparations.
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No difference was found between the activities of these two preparations.
Based on protein staining, there are no significant differences between the composition of these two preparations.
The absorption difference spectrum between the two PSI LHCI particles (Fig. 1a, green) matches the average absorption of reconstituted Lhca2 and Lhca9 (Fig. 1a, blue), indicating that the presence/absence of these two complexes is the main difference between the two preparations.
Four of the 18 targets did not significantly differ between the two preparations.
This suggests that the Δfitness values between the two preparations are quite small.
For the remaining 30 bioactives, fitness values between the two preparations were highly similar (R2 = 0.93).
c 2D representation and the docking pose of the bioactive showing maximum difference in docking rank between the two preparations.
The results of the statistical analysis proved the pharmacokinetic parameters were no significant difference (P > 0.05) between the two preparations.
As observed from Fig. 3, a highly flexible bioactive ligand showed a maximum fitness difference between the two preparations.
We ended up with 13 decoys that showed a Δdocking rank between the two preparations above the cutoff.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com