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Selection of SPPs that were identified as polymorphic between these two parental lines using this method identified 18,237 SPPs.
In addition, the rearrangement mediated by large and short repeats between these two parental mtDNAs extensively existed.
The S288C and RM11-1a genomic DNAs were sent to Fasteris for re-sequencing by using Illumina sequencing technology to verify SNPs between these two parental references.
The low polymorphism observed between 'Ferjalou Jalousia®' and 'Fantasia' using AFLP markers was previously reported using RFLP and SSR markers [ 13] and was likely the consequence of the very low genetic distance between these two parental varieties [ 13].
Our plant materials have been used as elite breeding resources by a Korean company, and thus the genetic relationship between these two parental inbred lines is likely much closer than to the reference accession or the relationship between the two cabbages used by Liu et al. [ 25]; this close relationship likely underlies the relatively low number of SNPs we identified.
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Pollination experiments conducted over several years revealed that hybridization between the most common oak species in Europe, Q. robur and Q. petraea [ 19], and between a first-generation hybrid and these two parental species [ 20] is possible.
A number of well-defined regions of the 'Heritage' and 'Tulameen' linkage maps exhibited significant segregation distortion and in many cases these regions were conserved between the two parental linkage maps, indicating the presence of lethal or sub-lethal genes that are conserved in heterozygous form in both parental genotypes.
Previous studies have reported a differential methylation between the two parental alleles for some of these genes.
These two clusters differentiate the transcript abundance between the two parental genotypes.
These SNPs were merged and used to detect SNPs between the two parental lines (Table 2).
In eukaryotes, these DSBs are repaired during meiosis by homologous recombination between the two parental chromosomes [ 11, 67- 69].
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