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The pattern of gene expression profile was almost similar between these two mutant strains.
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While Bmal1 expression seemed to be less changed in Rev-Erbα−/−Per1Brdm1 double mutant mice compared to Rev-Erbα−/− single mutants, Cry1 expression was unchanged between these two mutants.
We chose to examine Toll-81 and RelE20 mutant discs because of the strong phenotype of RelE20 and the genetic interaction between these two mutants.
The difference between these two mutants is that ΔPN197 lacks part of the positively charged region while this area is intact in ΔN197.
We also observed major differences in expression of apoptotic markers between these two mutants.
FTIR DDS between these two mutants and WT also demonstrate the identical effects of both mutants in this region of the spectrum (see the Supporting Information).
This is an unusual phenotype that has been reported only for Fgfr2b −/− mice (Petiot et al, 2003) suggesting that the underlying mechanism is common between these two mutants.
All freshly generated mst2∆ epe1∆ cells formed very small colonies, suggesting a strong negative genetic interaction between these two mutants, consistent with high throughput epistasis mapping (Roguev et al., 2008; Ryan et al., 2012).
This difference in root, but not shoot, total P concentration between these two mutants highlights a fundamental difference between them; the lower shoot P in Atph1 8 compared to WT was not due to a lower P concentration in the root, as may have been the case in Atpht1 1 2, which is impaired in Pi uptake.
Plants lacking GPT2 expression exhibited a delay in greening, such that there was a gap of 30 32 h between germination and greening in these two mutant lines.
Our results highlight the overlap between presumed checkpoint targets in these two mutants, which have different arrest phenotypes and presumably different checkpoint signals.
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