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To inquire the difference between these two isoforms using these two pA sites, qRT-PCR (quantitative reverse transcription real-time polymerase chain reaction) was performed and demonstrated that transcript using the distal pA site (with longer 3′UTR) was less stable than the shorter one upon transcription blocking (Fig. 1C).
The high homology between these two isoforms would suggest redundancy of their function, but recent studies have suggested different regulatory roles.
As fgaB and fgaC are almost identical, and share their regulatory regions, we sought to compare fgaA and fgaB expression patterns, and to explore whether functional differences occur between these two isoforms.
The molecular weights of AtxA and AtxB predicted from their sequences differ only by two Da, and therefore C-terminal sequencing is required to differentiate between these two isoforms.
However, our studies showed that only full length VHL but not VHL19 significantly interacts with PIASy, suggesting that there are distinct biological functions between these two isoforms of VHL and that PIASy association is most likely a critical one.
Although AQP4 antibodies have now been analyzed in several cohorts of NMO patients worldwide and the importance of AQP4 OAPs has been demonstrated in all of these studies, it is not clear whether the specificity and sensitivity of the antibody response to AQP4 differs between these two isoforms.
Similar(45)
Our suggestions may appear speculative, but we consider that the significant negative correlation of VEGF-C and VEGF-D with the HOMA index points to the possible relationship between down regulation of these two isoforms and insulin resistance development in morbidly obese subjects.
In the context of PKD1 and PKD2, which are highly homologous, most antibodies generated against these kinases cannot discriminate between the two isoforms.
In conclusion, these results reveal a functional difference between the two isoforms.
An abnormally high INSR-A INSR-B ratINSR-A INSR-Bcells of patINSR-A INSR-Botonic dystratio appears to be responsinle for the IR seen in these patients, further highlighting the functional differences between the two isoformusclethe recellsr.
Since the inhibition of SteC-dependent F-actin bundling was not complete, we carried out RNAi of Myosin IIA in these cells, to investigate the possibility of functional redundancy between the two isoforms.
More suggestions(16)
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