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Although initial analysis of background substitutional patterns using DNAREP1_DM did distinguish between elements in high- versus low-recombination areas of the genome, there were no significant differences revealed between these two genomic locations [ 15].
It is hoped that concordances between these two genomic approaches will help to identify 'driver' genes involved in tumour progression, rather than just differential 'consequences' in gene expression.
Synteny analysis between these two genomic regions revealed strong conservation of the moxXY genes forming a putative operon with moxFJGIR, thus suggesting that MoxYX might control A. lipoferum 4B methanol metabolism.
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Content variation between these three genomic domains was not restricted to LINEs.
Phylogenetic analysis of both the HA and the PB1 genes showed that they shared similar clustering of these Taiwanese isolates, suggesting that no obvious reassortment occurred between the two genomic segments.
Transformants are readily obtained in which tandem copies of the vehicle plus insert are inserted between the two genomic border sequence homologues.
Ten cDNA sequences, however, appeared to be intermediate between the two genomic clusters (Figure 4).
Of 28 samples sequenced in gag and env genes, 6 (21%) had discordant subtypes between the two genomic regions, as observed with recent studies in Nigeria [16], [20].
No significant differences in the frequency of recombination events were detected between the two genomic regions studied (9.1% E1-E2 and 9.6%% NS5A, Table 1) for the treatment-naïve, HCV-monoinfected patients group (HCV 0).
The lines show epistatic interactions between the two genomic locations at both ends.
The mCherry cassette from KP1272 was then inserted in-frame between the two genomic fragments using an engineered NotI site.
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