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Mating preferences may be tested using either no-choice or choice tests, but explicit comparisons between these two experimental paradigms remain limited.
We found a significant interaction between these two experimental factors on the development of immune defences and growth rates.
Since a major difference between these two experimental readouts is the time following induction of receptor expression (6 hours for the PFUS1-luciferase reporter system vs. up to 7 days for the plate growth assay), we decided to measure PFUS1-luciferase activity after prolonged incubation (24 hours).
We observed no significant difference (unpaired t-test, p > 0.7549) in the fraction of nematodes that reached adulthood between these two experimental conditions (mean ± s.e.m.: 91.6% ± 1.7% developmental success on OP50, n = 263; 92.3% ± 1.6% on 536, n = 262).
This discordance between our NMD targeting data collected in whole animals vs. data from cell culture experiments is likely a reflection of the tissue, physiological, and developmental stage differences between these two experimental setups.
Our results also clearly show that variation in cis-regulatory elements is the major mechanism that accounts for the majority of variation in MD genetic resistance between these two experimental lines, which supports the hypothesis that phenotypic variation in traits is mainly due to changes in regulation of gene expression rather than protein composition.
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Using a 5% significance level two-tailed testing of differences between two independent groups (usual care and intervention), a sample of 100 patients from the intervention and 100 patients from the control phase of the study would have 80% power to detect group differences between patients under these two experimental conditions of 0.40 SDs for the primary outcome measure.
A significant variation in the microbial community was observed from phase I to phase III, as indicated by the relatively low percentage of similarity (50%) between the fingerprints of these two experimental phases.
An interaction effect was observed between the incubation time at these two experimental temperatures regarding enthalpy values and the melting interval of the samples.
Interestingly, the only factor that varied between these three experimental runs was the initial pH value (X2; Table 2).
Through these two experimental paradigms, the relationship between Body Dysmorphic Disorder and these relationships was assessed.
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