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Assessment of the relationships between these molecules using the IPA knowledge base illustrates that among the highly predictive genes we find a small network of molecules that were both directly and indirectly functionally related.
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To study the ability of mutant receptors to recruit β-arrestin2, we co-transfected C-terminally Luc-tagged AT1R constructs with N-terminally GFP2-tagged β-arrestin2 and studied ligand-induced association between the molecules using a BRET assay.
Modeling interactions between such dissimilar molecules using molecular dynamics (MD) is an area of current interest.
By modeling the interactions between atoms and molecules using Newtonian principles, hypotheses about chemical or biological properties of certain molecules as they are interacting with the environment can be tested.
We extend our study to analyze common chemical substructures shared between biologically active molecules using a Maximum Common Substructure (MCS) approach.
These studies aimed at identifying different actin pools by distinguishing between populations of actin molecules using photoactivatable fluorophores and FRET, respectively.
I spent my time in the computer center carrying around boxes of Fortran cards, trying to do calculations of collisions between simple atoms and molecules using quantum mechanics.
Ubiquitin-chain elongation was quantified by HTRF® (excitation at 320 nm, emission at 620 and 665) between adjacent Flag-ubiquitin molecules, using anti-Flag-cryptate and anti-Flag-XL665 antibodies (CisBio) using a dedicated fluorescence plate reader (RubyStar, BMG Labtech, Offenburg Germany).
Finally, we can discriminate between corroles and other fluorescent molecules using spectral [ Fig. 5(H)] and florescence lifetime imaging [ Fig. 5(I)].
Connections were made between differentially expressed genes and the additional molecules using default IPA options.
During photosynthesis, plants and green algae absorb light in the form of photons and, through a complex series of energy and electron transfers between molecules, use the light energy to convert carbon dioxide to sugars.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com