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Furthermore, the reaction between the yellow solution and aliphatic bromide is investigated.
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The yellow solution was stirred at RT for 90 min.
The yellow solution was heated to 85 °C for 2 h.
The yellow solution was cooled and extracted twice with EtOAc.
Within few minutes, the yellow solution turned red.
Upon addition of the reagents, the yellow solution turned to a suspension.
THF (10 mL) was added, and the yellow solution was cooled to −78 °C.
The yellow solution was stirred for 30 min and then placed in a freezer (−28 °C) overnight.
The yellow solution was stirred for 20 min at room temperature.
The absorbance of the converted purple formazan crystals from the yellow solution was measured at 595 nm.
The yellow solutions were then cooled to room temperature.
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