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We also severed connections between the wing margin and the cuticle at about 22 hAPF.
Dumpy::YFP is present in the space between the wing margin and the cuticle.
(H ) Shows the pattern of Dumpy::YFP connections between the wing margin and cuticle these extend around the wing margin except in the posterior/proximal regions.
To disturb connections to the overlying cuticle without damaging the wing epithelium we disrupted apical extracellular matrix between the wing margin and the cuticle shortly before the onset of phase II, when this region becomes accessible (see Videos 8, 9).
Wings were pierced with a finely sharpened tungsten needle (cat. no. 501317; World Precision Instruments) at a site next to the small anterior eyespot, approximately halfway between the wing margin and the normal location of the eyespots.
(C – F ) Show 32 hAPF wings that were unperturbed (C ) or subjected to laser ablation at 22 hAPF (D – F ). Ablation of the connections between the wing margin and the cuticle were performed in different regions, indicated by blue dashed lines in (D – F ), and lead to altered wing shapes at 32 hAPF compared to the unperturbed control (C ).
Similar(54)
Landmarks 4 and 5 tended to move away each other, stretching the wing margin between longitudinal veins III and IV.
One such boundary occurs between the wingless (wg -expressing cells of the wg -expressingd the adjacellsprofeural cells, which give rise themargin sensory bristles.
Our proxy for wing width was the length of the line between the intersection of the L1 and L2 veins on the wing margin and the intersection of the L5 vein and the wing margin.
Table 2 also summarizes interactions between the EP modifiers and a strain that drives truncated Mastermind across the wing margin (C96-MamH), which creates a Notch pathway LOF phenotype.
Wings with well-developed lower calypter; axillary vein strongly curved towards the wing margin; hypopleural bristles absent; anal cell short and closed.
More suggestions(13)
between the maximum margin
between the timing margin
between the wing bone
between the delay margin
between the lung margin
between the interior margin
between the photoreceptor margin
between the wing disc
between the wing center
between the wing nut
between the wing biopsy
between the wing fin
between the test margin
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