However, for species/strain-level resolution, either longer ribosomal sequences or additional experiments are required [52], unless there are distinct and identifiable differences between the variable regions of the particular organisms of interest.
For example, both of the two known SNPs present between the variable regions of γ intimin in O55:H7 and O157 H7 [ 45] are located in the middle region of the 70-mer probe for eae.
The insertion of monomeric fluorescent protein derived from either Discosoma or Aequorea in-between the variable regions of anti-p185HER2-ECD anti-p185HER2-ECD anti-p185HER2-ECDVH/VL interfantibodyractions to create soluble coloured antibodies each with a single binding site, with isoelectric points of 6.5- 6.
A second generation DNA microarray could be developed for this purpose based on the variable regions identified between a number of sequenced strains, and utilise printed rather than spotted microarrays.
and Medicago or Lotus, finding that retrotransposon-rich regions are distributed in alternating blocks across these legume genomes and that these are interspersed between syntenic blocks and correspond to the variable regions which do not show synteny.
Of the variable regions, 23 Mb are located on chromosome 3 and by using a DA/O.chr3DA/K congenic strain these regions were excluded as important in arthritis regulation [ 13], and thus the regulatory effect should come from the remaining 15 Mb variable regions between DA/O and DA/K.
Among the 25 tracts detected in C. jejuni strain 07001, 6 were found in the CPS region, 6 in the FM region, 1 in the type II restriction-modification enzyme region and 3 tracts in the intergenic region between genes located on the variable region on the genome.
Species-specific oligonucleotide probes (S-SONP) for canine taeniid cestodes were then designed manually based on the variable region between the conserved primers.
Three putative H-NS binding sites were also identified in the EPV1 genome adjacent to genes gp33, gp40, and gp48, the first of which falls in the variable region between the two EPV1 strains (Figure 2).
These included the N domain in the N-terminal conserved region (amino acids 1 to 117), the PHD domain in the C-terminal conserved region with a PHD finger (amino acids 197 253), and the V domain in the variable region between the N and the PHD domains (amino acids 118 196).
The map of Pfam motifs in the variable region between the MT and HEL domains in Flexiviridae 2 polyproteins shows that they have a very similar domain organisation, basically an AlkB domain followed by an OTU domain and a peptidase domain, located towards the C-terminal part of the sub-sequence.
