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Restriction analysis of plasmid DNAs showed different patterns between the two transformants, EA76-T and KP76-T (Fig. 1b).
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The specific activity of CphA in the two transformants was 1.5 mU/mg protein.
Furthermore, CGP only accumulated in one of the two transformants which displayed enzyme activity.
The two transformant strains harboring rbcTk displayed growth under photoautotrophic and photoheterotrophic conditions, both dependent on CO2 fixation.
Unexpectedly, however, there were no mutations shared between M184 and the three transformants, indicating that none of the 27 mutations found in M184 were the cause of patAB overexpression.
Data represent the mean of averaged values for the three transformants ± SD.
Transfection efficiency was comparable in the three transformants (Fig 3D and E).
The three transformants were serially diluted and spotted on selective nutrient agar with glucose or galactose.
Further deletion to position −780 (pled05) led to failure to produce detectable GUS activity in any of the fifteen transformants tested indicating the presence of important positive regulatory information in this region between −780 and −756.
Fifteen of the seventeen transformants were shown to contain both the aphVIII gene and the g-luc gene.
Considering the higher initial LNZ MIC of 1974 compared to R6, the fold-increase in resistance was the same between the last two transformants (Table 1).
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