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Although a functional relationship between the two transcripts has not been demonstrated, the ATXN8OS CUG transcript has been suggested to regulate KLHL1 through an antisense mechanism39,40, with further speculation that pathogenic SCA8 repeat expansions lead to KLHL1 dysregulation.
Our data show varying expression ratios between the two transcripts of the same locus in the F1.
The only difference in sequence between the two transcripts is that the JM-a isoform is characterized by the insertion of a 75-nt sequence derived from exon 16, whereas JM-b is encoded by the alternative 45-nt sequence.
This makes it very hard to distinguish between the two transcripts when measuring gene expression.
For each gene we calculated the log ratio of the expression values between the two transcripts across all the samples.
RNAi constructs were designed to silence both isomers while real time primers did not distinguish between the two transcripts.
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For gene FGSG_00303 (encoding a transcriptional elongation regulator), the expression ratio between the two transcript isoforms is independent of the growth stage, whereas this ratio (reference isoform/alternative isoform) increased for gene FGSG_06760 (encoding a HMG box protein) after 8 h.
We eliminated any redundancy between the two transcript sets by selecting the longest transcript between any two transcripts represented in both files.
Table 1 shows the number of UniGene IDs identified by microarray cDNA, 454-sequencing and the overlapping UniGene IDs between the two transcript-based technologies.
The expression of genes FGSG_05122 (encoding a FAD dependent oxidoreductase) and FGSG_04141 (encoding a DNA repair protein) show strong developmental regulation and the expression ratio between the two transcript isoforms changes strongly at different vegetative growth stages.
Eight out of 18 rehabilitation providers reported professional experience with sleep medicine or sleep health hygiene; only 2 had sleep medicine training.> In this study, the inter-rater ICC value was 0.90, indicating excellent inter-rater reliability between the two transcript coders.
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