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The phrase "between the two subgroups" is grammatically correct and can be used in written English.
Example: The experiment showed a significant difference in behavior between the two subgroups, with one group exhibiting more aggression and the other showing more empathy.
Exact(60)
No significant difference in MD was observed between the two subgroups (Figure 5b).
Sleep latency, nMMA and TST did not differ significantly between the two subgroups.
No significant differences were observed between the two subgroups.
There was no difference between the two subgroups of patients.
There was a strongly significant difference of the B-glucose between the two subgroups (p < 0.0001).
Intensity and duration of MO-attacks were not different between the two subgroups.
Also, glomerular involvement differed strongly between the two subgroups.
Survival curves were compared between the two subgroups.
Correspondingly, the expression trait value would also be different between the two subgroups.
However, there was no difference in the levels of sEng between the two subgroups (figure 3a).
Maternal age and BMI were not significantly different between the two subgroups.
More suggestions(19)
between the two subclasses
between the two populations
between the two subsets
between the two subpopulations
between the two sub clusters
between the two fines
between the two kings
between the two institutions
between the two stories
between the two things
between the two cases
between the two countries
between the two cops
between the two negotiators
between the two cities
between the two worlds
between the two programs
between the two pictures
between the two municipalities
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com