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Aiming to restore the imbalance between the two splice products, antisense oligonucleotides (AONs) were employed to induce exon P3A skipping.
5) Average differences in score between the two splice sites from the two alleles of each sdSNP.
Expression of livin α and β mRNAs was analysed by RT PCR, using primers which distinguish between the two splice variants (Crnković-Mertens et al, 2006a).
Although many genes were similarly regulated between the two splice variants some were found oppositely regulated, indicating different functional roles of TXNRD1_v2 ascribed to the N-terminal domain of that protein.
For example, the interval between the two splice site signals MDN-IR and MA1-IR represents a retained intron between an upstream exon with multiple functional donor sites and a downstream exon with multiple functional acceptor sites.
For 'heat shock cognate 71 kDa protein', the 7 unique peptides that we identified can not distinguish between the two splice isoforms 1 or 2. However, isoform 2 has a proposed function as an endogenous inhibitory regulator of HSC70, via competition with co-chaperones.
Similar(54)
The energy dissipation capacity of the beam splice with a reduced beam section is between the two splices.
We assigned immunoblot bands to individual SFK species by testing antibodies against lysates from BMDMs derived from WT, Hck −/−, and Lyn −/− mice (Lowell et al., 1994; Chan et al., 1997) and were able to differentiate between Hck, Fgr, and the two splice forms of Lyn, LynA (56 kDa) and LynB (53 kDa) (Stanley et al., 1991; Yi et al., 1991).
When comparing the levels of SRp20 with SRp30c, the actual difference between these two splicing factors was 1.6-fold.
Unlike the AA class where a G(T nG consensus sequence was observed, there appears to be no clear consensus in the intervening sequence between the two donor splice sites.
The T cell-specific effect of locally administrated glucocorticoids might reside in the imbalance between the two alternatively spliced transcripts of the GR that have been suggested to have different functional characteristics.
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