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Environmental features differ dramatically between the two spawning habitats, creating divergent selection pressures on early life history stages.
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On the night of 27 July between 22 30 and 23 30 h three C. echinata and five C. crassa spawned revealing that the three spawning C. echinata were female, while four C. crassa were females and one was a male.
Mantel tests for one SNP set ('Outlier 2') strongly suggested that variable timing of spawning, particularly among populations of the three spawning-habitat types (see summary in additional file 3), may be the most important barrier to dispersal between these populations.
We collected sperm from spawning male colonies using 60 mL and 500 mL plastic syringes, aiming for areas in the water column near spawning colonies where sperm density was visibly high, such as in the valley between two spawning pillars.
Each breeding trial consisted of 1 4 spawning events; however, a maximum of the first two spawns per trial (referred to subsequently as spawn A or spawn B) were included in the behavioural analyses.
"Ninety per cent of the biomass in this river is in two spawning spots," he says.
The Bay of Piran dispute has spawned fierce words between the two countries, with each accusing the other of a needless territorial grab.
In the brief interview, they discussed collaborations, art direction, and how working on Beyonce's "Formation" Tour "spawned a brand new relationship" between the two of them.
Recent interest in "overlapping syndromes" in general – but more specifically the concept of overlap in frailty and depression – has spawned reviews indicating a positive association between the two.
But the power-sharing agreement between the two main factions that paved the way to the national dialogue has subsequently spawned a form of paralysis.
The trip, which spawned the phrase "ping pong diplomacy" is often credited with reviving diplomatic relations between the two countries.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com