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With probes designed based on the internal transcribed spacer sequences of the rRNA genes of fungal and oomycete strains, we produced a macroarray, which included five types of oligonucleotide probes: monomers (20 24 nt), dimers (40 48 nt), dimers with a poly-A spacer of 10 bases between the two repeats (50 58 nt), monomers with a poly-A tail of 10 (30 34 nt) and 20 (40 44 nt) bases.
Here an association between the two repeats was also required; separating them as in LigB7'-9 and LigB10-12 significantly weakened the activity observed with intact LigB7'-12 (Figure 5B).
bnumber of mismatches between the two repeats.
Whereas gene conversion transfers information between alleles, DRR results in the deletion of information located between the two repeats (Agmon et al. 2009).
In the T state, by contrast, two narrow water wires form on the periplasmic face of the TM domain, again reaching into the proton-binding site, and approximately along the interface between the two repeats R1 and R2.
Another few minor differences between the two repeats as reported in the genomic DNA sequence NC_005364 could not be confirmed by resequencing these loci and this might be due to initial sequencing errors.
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Although no gold standard measurements of X a and X s via a validation subsample are available for this circumstance, the discrepancy between the two repeated measurements allows us to estimate the misclassification probabilities under certain assumptions.
The differences between the two repeated SUVmax readings at 60 min were all less than one in absolute terms, apart from those of patient no. 3, 5, 10, 23, 26, and 42 (Fig. 1).
The highest degree of sequence conservation of monomeric beta-satellites among both species was always found between the two repeat blocks in orthologous position within the DUXY locus.
Fig. 9 indicates that our fold prediction methods of choice allow to distinguish between the two repeat types and we have observed that this applies generally.
There is only a single copy of the sequence in other Subcluster C1 phages, suggesting either that all of the Subcluster C1 phages have lost a 1.35 kbp segment via recombination between the two repeat copies, or alternatively that LRRHood acquired this DNA segment by recombination with a second phage partner of unknown origin (Fig. 7B).
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between the two repetitions
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