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The highest degree of sequence conservation of monomeric beta-satellites among both species was always found between the two repeat blocks in orthologous position within the DUXY locus.
Fig. 9 indicates that our fold prediction methods of choice allow to distinguish between the two repeat types and we have observed that this applies generally.
There is only a single copy of the sequence in other Subcluster C1 phages, suggesting either that all of the Subcluster C1 phages have lost a 1.35 kbp segment via recombination between the two repeat copies, or alternatively that LRRHood acquired this DNA segment by recombination with a second phage partner of unknown origin (Fig. 7B).
In the third step of TREC-IN, the whole cassette flanked by the two repeat sequences is removed via homologous recombination between the two repeat sequences.
A clear gap was observed at the CL14-CL34 junctionsome some fibers, suggesting the presence of other sequences at the junction between the two repeat arrays.
Between the two Repeat A/B regions, multiple Repeat C regions exist in tandem, but the number of Repeat C regions varies between MITE464 copies, up to 14 repeats.
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Although no gold standard measurements of X a and X s via a validation subsample are available for this circumstance, the discrepancy between the two repeated measurements allows us to estimate the misclassification probabilities under certain assumptions.
The differences between the two repeated SUVmax readings at 60 min were all less than one in absolute terms, apart from those of patient no. 3, 5, 10, 23, 26, and 42 (Fig. 1).
With probes designed based on the internal transcribed spacer sequences of the rRNA genes of fungal and oomycete strains, we produced a macroarray, which included five types of oligonucleotide probes: monomers (20 24 nt), dimers (40 48 nt), dimers with a poly-A spacer of 10 bases between the two repeats (50 58 nt), monomers with a poly-A tail of 10 (30 34 nt) and 20 (40 44 nt) bases.
Here an association between the two repeats was also required; separating them as in LigB7'-9 and LigB10-12 significantly weakened the activity observed with intact LigB7'-12 (Figure 5B).
bnumber of mismatches between the two repeats.
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