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However, whereas this short sequence between the two reading frames can be translated in cattle, it contains a STOP codon in human and mouse.
The incremental cost of single reading with CAD versus double reading was estimated assuming both a 0.3 and 0.8 percentage point difference in recall rate between the two reading options [ 12].
This view is supported by Coultheart and Rastle [ 11], who already stated in the original description of their reading model that initial processing stages are shared between the two reading routes (direct and graphophonological).
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The two reads from each cluster were merged providing the adapter sequences and requiring at least an 11nt overlap between the two reads.
The program first identifies perfect overlaps between the two reads, and then finds reads that overlap if a single mismatch is allowed.
For paired-end reads, all positions between the two reads were considered as transcribed.
The fragment size determines the distance between the two reads, often called the 'insert' size.
The plot shows that there are no systematic differences between the two reads.
By independent we mean that any overlap between the two reads is purely by chance.
When the credibility between the two reads was less than 98% a third sequencing assay was performed.
The forward and reverse sequence reads were assembled and conflicts between the two reads were resolved using the SEQMANII program (DNASTAR, Madison, WI).
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