Sentence examples for between the two primer from inspiring English sources
The first IAC with a size of approximately 200 bp was constructed by deleting a part of the amplicon of the original target DNA (500 bp) between the two primer sites to produce an IAC smaller than the target DNA.
In short, we did not observe any significant differences between the two primer sets.
This assay uses a specific oligonucleotide probe, annealing between the two primer sites, which is labeled with a reporter fluorophore and a quencher.
*Considering 162 single expected product and 96 single larger product whit amplicon size <500 nt Primer pairs that amplify a larger than expected products are presumed to be the result of an intron between the two primer sites.
The final results of amplification of the target products indicated no obvious differences between the two primer types, i.e. they presented similar sizes of products on the agarose gels (data not shown).
The most likely cause for the lack of amplification in the dwarf lines is either a single-nucleotide polymorphism or deletion at a primer site that prohibits primer extension or the presence of an insertion in the region between the two primer binding sites that extends the fragment to be amplified beyond the length limit of a typical PCR.
