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We further selected candidate miRNAs that had a CT value of <30 in at least one pool and showed more than four-fold altered expression between the two pooled samples.
The loci retained after quality control filtering were ranked by Weir and Cockerham's45 FST between the two pooled reference groups (wild and domestic salmon), and the 95 most informative loci for which suitable assays could be developed were incorporated into the custom Fluidigm EPI array (see below).
Before conducting the relative protein profiling analysis between the two pooled samples from VKH patients and normal controls, several quality control measures were performed on the replicates of both pools to determine analytical reproducibility.
The results in Table 2 show the peaks that are detected as significantly different between the two pooled samples (here the P-values do not reflect biological but simply experimental variation).
To evaluate sensitivity of our method, the significance level of the differences in the allele frequencies between the two pooled samples with the closest allele frequencies (Table 3) was assessed by the Kolmogorov-Smirnov test.
Overall, the two analyses are in close agreement, but one of the differences between the two pooled analyses is in the exposure metric used: Ahlbom et al. (2000) examined the association between the geometric mean ELF level and childhood leukemia in 9 epidemiologic studies, whereas Greenland et al. (2000) used the arithmetic mean to examine this association in 12 studies.
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The stage for the event sat between the two pools.
If you modify palloc.c as part of your frame table implementation, be sure to retain the distinction between the two pools.
This quick visualization also showed me that the connections were not evenly balanced between the two pools; indeed, they were heavily concentrated in one of the two pools.
In a study of the differences between the two pools of students over the last five years, he added that submitters averaged 1219 on their SAT's and non-submitters 1003.
The technique exploits differences in methylation between the two pools of genetic material.
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