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Results for Pat208 required manual curation due to the PhyloWGS algorithm's propensity for designating p.Leu13fs as the parent of p.Ser14fs, in violation of the "crossing rule" outlined in the PhyloWGS paper as well as the ample evidence for a sibling relationship between the two mutations.
The exponential distribution of the spacing between the two mutations in EGFR and TP53 doublets in the same exons (R2 = >0.98) is highly unlikely by chance.
This may be because that the high correlation exists between the two mutations and the other mutations, especially the mutation of glutamic acid.
A simulation was performed previously for each of the TP53 exons 5 9 to test the null hypothesis that the mutations in TP53 doublets are independent events and that the distribution of the spacing between the two mutations depends only on the spectrum of mutations in the TP53 gene and the size of the mutation target [13].
For simplicity we assume there is no direct or indirect (allostery) interference between the two mutations.
Double heterozygous Stat5abKO/ Stat3KO mice were indistinguishable from Stat5abKO/+ littermates arguing for a lack of interaction between the two mutations.
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Fisher exact tests were performed to determine significance of variable phenotypes between the two mutation groups.
The inherent differences between activating and deleterious mutations are perhaps the greatest contributing factors towards the relative inaccuracy in prediction quality between the two mutation types.
However, the relationship between relative gamete mutation load and male age differed considerably between the two mutation probability functions (Fig. 1E, F).
Any observed difference between high-quality SNPs and DIMs could potentially be a result of ascertainment biases between the two mutation sets.
Differences in the mutagenic efficiency and the (related) magnitude of response between the two mutation endpoints may be explained by several factors.
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between the two moves
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