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As the genome wide coverage increases the discrepancy in callable bases between the two library preparation methods decreases.
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Table 5 shows that there is a small overlap between the two libraries in terms of compounds, scaffolds and frameworks.
Only one compound is shared between the two libraries, and they both contain a large percentage of exclusive scaffolds and frameworks.
Finally, genes with a P value < = 0.05 were deemed to be significantly different between the two libraries.
Based on the normalized number of reads per sample (specifc miRNA/total sequencing tags in the library), the majority of miRNAs (256) were expressed approximately equally between the two libraries.
Genes were designated to be significantly differentially expressed if the p value was <0.001, and there was at least a 1.5-fold change in sequence counts between the two libraries.
We employed IDEG6 (http://telethon.bio.unipd.it/bioinfo/IDEG6/) to identify differentially expressed mRNAs based on their relative abundance which was reflected by total count of individual sequence read between the two libraries.
One hundred and sixty-seven pre-miRNAs overlapped between the two libraries.
Clustering analysis suggests virtually no overlap between the two libraries.
These were significantly different between the two libraries.
Meanwhile, the expression amounts of the miRNAs between the two libraries were compared.
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