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First, the biolayer interferometry data provide evidence of direct interactions between the two isolated enzymes.
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It was seen that there were not obvious variations in the size of rRNA gene products between the six isolated bacteria and the size of the 16S rRNA gene product of all isolated bacteria investigated in this study was approximately 1.4 Kb to the relative DNA size marker).
Dot plot analysis of ATV vs RRV sequences identified several polymorphisms between the two isolates.
Intriguingly, we discovered statistically significant and divergent levels of gene expression between the two isolates profiled for 64 genes.
Where this was so in the third, haploid × haploid test, a small mycelium piece from the junction region between the two isolates was sub-cultured on 2 % malt agar to help clarify the result from its appearance.
Six proteins (HaCR4, 9, 10, 11, 12 and 15) were absolutely conserved (100% identity) between the two isolates.
The VNTR's listed in Table 2 accounted for the difference in size between the two isolates.
The 24-locus MIRU-VNTR genotypes and spoligotypes were fully conserved between the two isolates within each of the eight pairs of serial isolates tested.
The results show no difference in nucleotide sequence between the two isolates except for the presence of SCCmec in the MRSA.
Both Plasmodium isolates were characterized by a unique DNA sequence for each of the cox1, cyt b and ldh markers, differing between the two isolates.
On the contrary, splenic and hepatic colony counts declined progressively in all mice to nearly undetectable levels, showing no significant differences between the two isolates (results not shown).
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