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Tests to determine this resolution were done by analyzing two images of the same worm (B01,B05) and detecting at which resolution the correlation (measured via NCC) between the two intensity images dropped significantly.
Hence, the scaled lowess fitness (LOcally- WEighted Regr ession) normalization algorithm was applied to minimize signal-dependent non-linear bias between the two intensity levels.
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Only moderate correlations between AMI and the four intensity-related parameters were found (median r = 0.41, all P < 0.05), indicating that frequency-related parameters alone may not suffice to determine behavioural importance for animal welfare.
The relationship between the two different intensity scales had to be verified.
The variability between two identical probes for the same transcript was evaluated using the ratio between the two probe intensity levels (fold-change, FC) as a measure of signal difference.
However if the time interval between the two fluorescence intensity measurements becomes too long then the daughter cells themselves would start to undergo mitosis which adds another layer of complexity to the analysis.
Signal intensities and their significance of the difference between the two signal intensities (p value of differential gene expression) were measured using the Feature Extraction software (version 8.1, Agilent Technologies).
A surface of prevalence is the ratio between two intensity surfaces (number of cases per surface unit), while a surface of relative risks corresponds to the ratio between the density surface (whose integral has been reduced to one) of positive cases and the density surface of control cases (population exposed to the risk).
The accuracy of EBVs was, nevertheless, equally high for R, S + IND and S + AVER, and these values did not essentially differ between the two selection intensities.
The values for each spot were shown as log2 ratios between the two signal intensities (M-values).
However, the difference in effectiveness between the two program intensities and the possible influence of the number of meetings remain to be studied.
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