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No significant differences in expression level were noticed between the two insertion sites.
The great variability in the expression patterns between the two insertion sites further suggests that the NP225 genomic region is highly susceptible to position effects, perhaps by presence of a natural transposon [25], and can be influenced by the regulatory elements of neighboring genomic elements.
We used the Kolmogorov Smirnov test to determine whether the shape of the frequency distributions between the two insertion types is significantly different.
The segregation of this complex region in combination with a CO in between the two insertion sites of the transpositions resulted in either a duplication or a deletion of the transposed sequence within individual offspring genomes.
However, we see striking differences between the effects elicited by charged residues flanking the TM segments when comparing the mitochondrial inner membrane and the ER, pointing to an unanticipated difference between the two insertion systems.
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Panels G and H (Bayesian analysis) and I and J (MP) show that there is a 10 20 percent difference in accuracy for a given level of gaps in an alignment between the two insertion-deletion ratios, whether for MD or BC treatments.
This indicated that recombination between the left and the right elements is likely to have caused a deletion of the 405 bp between the two insertions.
To test for a genetic interaction between Jhe and clt, the two insertion strains, Jhe e01859 and clt BG01317, were crossed to generate transheterozygous flies containing a single P-element insertion in each gene (Jhe e01859 +/+ clt BG01317).
Also the laryngotracheal expression (MACS1 enhancer) is drastically reduced between the same two insertion sites (arrowheads in Fig. 3A).
The results obtained validate the proposed optimisation method and we demonstrate a clear correlation between the two hole insertion criteria.
The dvps4Δ7b allele was generated by using P-transposase-based deletion by FRT-site recombination between the two transposon insertions f07379 id: AY515148 (WH p-element) and d09806 id: AY515149 (XP p-element) situated on opposite sides of the dvps4 gene locus [26] (a gift from Ruth Palmer) and Hs-FLP and P[w+]/FM7c, w−.
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