We obtained the expected results from 32 sets of primers between the two genotypes.
There was no significant difference seen in alanine aminotransferase (ALT) values between the two genotypes.
There were no differences in PaCO2, pH, and lactate between the two genotypes after high VT ventilation (data not shown).
We then conducted an RT-PCR analysis with leaf samples to compare band intensities between the two genotypes.
Many genes encoding transcription factors (TFs) were also differentially regulated between the two genotypes in response to salt.
REM sleep, however, was similar between the two genotypes over the 24 hours.
On semithin sections, no obvious difference could be observed between the two genotypes.
This difference between the two genotypes diminished again on day 60.
Swimming and contact righting did not show clear differences between the two genotypes (data not shown).
FASN mRNA levels were similar in skin between the two genotypes (Fig. 6H).
In untreated animals, only a few genes differed in basal expression between the two genotypes.
