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The enzymatic capacities were highly conserved between the two fructose types.
This higher sucrose concentration may explain that no difference in sweetness was found between the two fructose types.
Indeed, out of the twelve assayed enzymes, only five showed slight but significant differences between the two fructose types.
Differences between the two fructose types could arise from the systemic structure of the network controlling sugar metabolism.
Interestingly, a difference in the FK capacity was highlighted between the two fructose types in our study.
Could these subtle differences observed in the enzymatic capacities explain the difference in the fructose concentrations between the two fructose types?
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The fructose-to-glucose ratio was also stable between years for the two genotypes as monitored over two years (Additional file 2).> -wrap-foot> The fructose-to-glucose ratio average with standard deviation and minimal (min) and maximal (max) values for the two fructose types on the final sampling date.
At maturity, there was no overlapping of the fructose-to-glucose ratios of the two fructose types.
The mean, minimal and maximal values of the fructose-to-glucose ratios for the two fructose types are presented in Table 2.
The two fructose types showed similar time courses for metabolite accumulation, except for sucrose, which accumulated faster in the 'low-fructose-to-glucose-ratio' genotypes.
For all DE genes between U85 and T85, we identified four canonical pathways that were overrepresented between the two breeds: glycolysis/gluconeogenesis, fructose and mannose metabolism, the pentose phosphate pathway, and vascular endothelial growth factor (VEGF) signaling.
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