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Relative biomass growth, mortality, and net biomass change were calculated as the absolute value divided by the mean standing biomass between the two focal census periods.
The Doping IAT effect was calculated as the difference time difference between the two focal test blocks as shown in Figure 3.
The volume of the dissector (Vdis) was calculated by multiplying the area of the dissector grid (29,285 µm2) by the distance between the two focal planes, which were measured with a microcator (Heidenhain, Traureut, Germany) connected to the Z axis of the microscope stage.
The overlapping area between the two focal spots is then deactivated leading to a smaller net excitation area.
We follow up with functional annotation and analysis of genetic differentiation in gene expression between the two focal house finch populations studied here to identify genes exhibiting extensive differentiation between these populations.
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The relative codon bias between sets of genes in the two focal species, P. falciparum and T. annulata, was calculated based on the method developed by Karlin et al. [ 44].
To illustrate the differences between the double pulse pattern of the two focal species and the ancestral single pulse pattern, we compare the data from N. ensiger and N. maxillosus to that of a species with single pulse pattern (N. robustus).
Table S1 also provides covariances and correlations between the three focal traits and fitness.
Because the genetic, environmental and hence phenotypic correlations between the three focal traits were weak, we chose to use single trait models (additional file 1 Table S1).
EGFR amplifications were not statistically related with EGFR mutation but 5/13 in the EGFR group versus 1/11 in the KRAS group had estimated copy number > 4. Regardless of statistical difference between the two groups, focal amplifications and homozygous deletion are of special interest as they may indicate oncogenes or tumor suppressors.
By changing the distance and direction between two focal points, it should be possible to find the diffusion tensor in anisotropic media, since there is a spatial and temporal component in the analyzed cross-correlation.
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