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Clapp speculates that instead of relying on energy transfer between the two fluorescent dyes, you could just change their ratios and get the colors you want.
If a second dose is given after 11 14 days, the amount of bone formed during that interval can be calculated by measuring the distance between the two fluorescent labels.
In brief, an ICQ of 0 indicates random staining between the two fluorescent
Any bias in signal intensity between the two fluorescent dye channels in a microarray was normalized by locally weighted linear regression analysis or Lowess normalization [16] using MIDAS (freely available from http://www.tm4.org/midas.html).html
The crosstalk between the two fluorescent channels was below 0.1%.
The distance between the two fluorescent molecules was then tracked over time.
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In vitro experiments demonstrate a rapid and precise relationship between the ratio of the two fluorescent emissions and concentration of glucose in saline for the physiological range of concentrations (0 500 mg/dl) over seven weeks.
Distances between the brightest centers of the two fluorescent foci were measured in 3 dimensional reconstructions of the cells using Softworx (DeltaVision, GE Healthcare, Pittsburgh, PA).
Tumour coverage of each of the four fluorescent markers was calculated as the ratio between fluorescent pixels and the total number of pixels within the ROI.
By using FRET, changes in the intermolecular spacing between the two different fluorescent probes labeled emulsifier were characterized.
Using an Agilent Bioanalyzer, typical indications of high quality, intact total RNA samples are electropherograms with flat baselines and a relatively flat valley between the two strong fluorescent rRNA peaks.
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