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In order to verify that the increase in GFP intensity was not due to an artifact we obtained the Pearson product moment correlation coefficient (CC) r, a dimensionless index that ranges from −1.0 to 1.0 inclusive, which reflects the extent of a linear relationship between the two fluorescence intensity data of GFP and mRFP on acceptor bleaching.
In order to verify the increase in GFP intensity due to any possible artifact we obtained the Pearson product moment correlation coefficient r, a dimensionless index that ranges from −1.0 to 1.0 inclusive and reflects the extend of a linear relationship between the two fluorescence intensity data of GFP and mRFP while bleaching.
This is not only true of the comparison between the photographic scores and the fluorescence imaging but also between the two fluorescence imaging techniques.
The distance between the two fluorescence peaks is about 200 nm to 300 nm which would allow small organelles to pass through.
In order to reduce variance between the two fluorescence imaging techniques it was necessary to utilize the same masks of the teeth.
Aliquots of BC also were immunostained with a single antibody and used to establish the appropriate compensation for signal overlap between the two fluorescence channels and to establish appropriate, conservative demarcations of fluorescence positive.
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Briefly, three lines were drawn across each cell to exclude the nucleus, and the Pearson correlation coefficient (r) was computed for the relationship between the intensities of the two fluorescence channels (green for IP3R fragments and red for calreticulin).
LSM software was used to calculate the ratio between the two emission fluorescence values from selected well-loaded regions in each islet.
Genes that are expressed at the same level in both sexes will have equivalent amounts of transcript bound to probes and so the signal will be a combination of both Cy3 and Cy5 signal thereby generating a signal intermediate between the two (yellow fluorescence).
This brings the two fluorescent proteins near each other, causing fluorescence that can be seen with a microscope.
In this paper, there is a comparison between the advantages of two fluorescence approaches of commonly available and affordable instruments: the microplate reader (MPR) and the flow cytometer to detect the P-gp efflux activity using calcein-AM.
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