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The phosphate group at the splice junction between the two exons originates from the 3' splice site at the precursor.
This weight represents the correlation between the two exons' inclusion rate profiles.
In this respect, the fern ndhB gene provides a unique opportunity with its intragenic location change between the two exons.
Using single cell qPCR on the Biomark HD, a concordance rate of ∼85% was seen between the two exons.
Specific primer pairs were placed in different exons and a dye-labelled probe was placed over the boundary between the two exons in the amplicon.
The Tukey HSD test (JMP 9.0) was used to evaluate differences of the ratios of dN and dS between the two exons among different exon copy number types.
Similar(39)
The two exons found between exons 7 and 10 in human AMBN were numbered 8 and 9a; therefore, the extra exons found between exon 9a and 10 were numbered 9b, 9c, etc.
If the distance between two exons was less than 10 bases, the two exons would be merged together.
The differences between the three exon clusters regarding their evolution are likely caused by different selection pressures on the exons.
We numbered the four exons conserved between human and mouse as exons -1, -2, -3 and -4 (in order of increasing distance from the translation initiation site).
The major difference identified between these two exons is that exon 8 has a weaker 3' splice site and a polypyrimidine moiety that contains several mismatches from the consensus sequence (Figure 2A, TGTTCTAG ca), whereas exon 9 has stronger 3' splice site which has conserved consensus sequences (Figure 2A, TTTTCTAG gc).
More suggestions(17)
between the two cases
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