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The model involves two linked equilibria: association between the two DNA fragments and binding of the protein exclusively to the complex between the two DNA fragments.
In all, 209 recombinants between the two DNA markers were selected from 1,190 F2 plants.
In this study, both HWA1 and HWA2 were located between the two DNA markers on chromosome 11: KGC11M1 and RM27122.
Here, we characterize a less explored layer of the mutational landscape of cancer: mutational asymmetries between the two DNA strands.
Kinetic data identified the association between the two DNA half-sites as the rate-limiting step of the assay.
This fractures the connection between the two DNA molecules resulting in the formation of nicked linear duplexes and can be repaired by DNA ligases [30].
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Our newly designed nucleases, Sp1(P1)GLI and Sp1(P1G)GLI, can strongly bind to a lanthanide ion and show a unique DNA cleavage pattern, in which certain positions between the two DNA-binding sites are specifically cleaved.
The HDX data show that 4-HPA binds between the two DNA-binding lobes of NadR and stabilizes this state with little conformational perturbation.
All three alleles were detected when the DNA were mixed, irrespective of the ratio between the two DNAs.
To see this, it is illuminating to consider the famous experiment that is said to have enabled a choice between the three DNA replication mechanisms in 1957.
In order to assess the technical reliability of deriving allele frequency estimates from the DNA pools, we compared RASav for the three technical replicates within each DNA pool and between the three DNA pools.
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