Sequencing depth was comparable between the two cell types displayed.
Further, the transwell migration assay was performed to determine the differences between the two cell lines.
Control groups included constructs where direct contact between the two cell types was prevented.
The results reveal key differences in methylation patterns between the two cell types, suggesting potentially different gene regulation mechanisms in pluripotency and differentiation.
Besides these global similarities between iPSC-Mac and PBMC-Mac, direct comparison between the two cell types revealed several differentially expressed genes (DEG) (Fig. 2e).
Together, these findings suggest that the DCL and EVL establish adhesive contacts and that these contacts can transmit traction forces between the two cell layers.
Signaling between the two cell lines helped to prevent matrix degradation and maintained stable networks for a maximum of 7 days.
A subset of these sequences was subsequently confirmed to have differential expression between the two cell types.
*indicates significant difference between the two cell types (p < 0.05).
MET and EMT CSCs can readily transition back and forth between the two cell states.
A two-sample t test was also performed between the two cell lines at each density.
