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Based on this analysis, 3 out of 20 deletions showed extensive sequence identity between the two breakpoints, strongly suggesting that NAHR was the mechanism of their formation.
No homology was found between the two breakpoints.
The variants that have more than 85%% identity between the two breakpoints are annotated as non-allelic homologous recombination (NAHR).
Complex genomic rearrangements - Both fusion points are around genomic breakpoints with multiple segments between the two breakpoints, see Figure 4F.
The estimated reference depth (R) is taken from the average number of spanning reads between the two breakpoints,.
These markers show a deficiency of LD between the two breakpoints, despite high levels of intralocus LD.
Similar(52)
Once the new limits for the 14 breakpoint regions in D. mojavensis were established, we analyzed the similarity between the two breakpoint regions of each inversion using BLAST 2 sequences [ 64].
In both cases, each breakpoint harbors a copy of BuT5 and the exchanged TSDs between copies of the two breakpoints denote ectopic recombination as the generation mechanism (fig. 1).
The distances between each of these markers and the two breakpoints defining this conserved block in opossum were then rescaled onto the tammar wallaby version of this conserved block, to provide an estimate of the location of this centromere in the tammar wallaby.
According to the array CGH data the two breakpoints are located between RP11 247C02 and RP11 672A20 (proximal breakpoint) and RP11 758J16 and RP11 745I11 (distal breakpoint).
According to our sequencing results, one of the two breakpoints is located between the fourth and fifth base pairs from the 3' end of MAP0008c (published coordinate 11,150).
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