On the other hand, we also observed distinct tumor-specific phenotypes between the two backgrounds.
This could lead to higher intraperitoneal bacterial count between the two backgrounds with equal fecal spillage.
Differences between the two backgrounds were also observed (Fig.5-B, F 1,53) = 34.05, p<0.0001), FVB mice being less active and habituating faster [background × bins: F 5,265) = 6.40, p<0.0001] than B6.
To determine the relevance of these in vitro findings, we performed survival studies between the two backgrounds in a purely infectious model of peritonitis.
Differences between the two backgrounds were detected in the overall levels of startle reactivity that were critically modulated by the intensity of the pulse stimulus: B6 showed stronger responses to the 100 dBA and lower ones to the 120 dBA pulses compared to FVB [background × pulse: F 2,112) = 61.1, p<0.0001, post hoc: p<0.05; Fig.4-B].
To determine if H-NS influences Hha-DNA interactions, we performed the same experiment in an isogenic hha/hns double mutant and found that the number of putative Hha binding sites decreased to 19 genes and 25 intergenic regions with 26 of these targets common between the two backgrounds and 18 were specific for the hha/hns mutant.
The logarithm of the ratio between the two background-subtracted averages of feature pixels in the cy3 and cy5 channels (log2[cy3/cy5]) was used as a measure of the difference in abundance of the corresponding DNA fragment in the two representations hybridized to the array.
The logarithm of the ratio between the two background-subtracted averages of feature pixels in the cy3 and the cy5 channel (log2 [cy3/cy5]) was used as a measure of the difference in abundance of the corresponding DNA fragment in the two representations hybridized to an array.
No significant difference was observed between the two backgrounds (data not shown).
For the continuous variable, the SMMS score, Student's t test was used, to study differences between the two backgrounds and between the two genders.
