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ChrX is the chromosome showing the largest difference between the two assemblies, with strikingly fewer assembled sequences in Btau than UMD (88.5 Mbp versus 148.8 Mbp, respectively).
Surprisingly, only 448 contigs are identical between the two assemblies based upon results of a BLASTN alignment [30].
The gene conversion characteristics were indistinguishable between the two assemblies (data not shown).
For the current work, our focus was on the differences between the two assemblies.
First, we used MUMMER Nucmer [ 64] to detect orthologous sequences between the two assemblies.
The average proportion of heterozygous genotypes per marker was comparable between the two assemblies except for chromosomes 2A, 3D, and 4A, where differences between the two assemblies were large.
Similar(39)
The highest value therefore was 0.5 for perfect overlap and the lowest value was 0 if no sequence was identical between the complete sequences of the two assemblies.
As shown in Table 3, the differences between the allelic counterparts in the two assemblies range from 0.22%to4.34%4% or 2.6 to 52.1 bases when translated into the number of differences for each 1,200 bp, reflecting considerably higher mutagenic activities and limited selection pressure in this region.
A striking structural similarity is seen when the central part of the M. maripaludis SepRS tetramer is superimposed onto the 2 heterotetramer of T. thermophilus PheRS: the r.m.s.d. between 525 corresponding Cα atoms in the two assemblies is only 2.2 Å [ 34- 38].
The similarities between the three assemblies (90, 98 and 90e) are illustrated in Figure 1 a Venn diagram which shows that 72.68% of the raw sequencing reads were integrated into contigs common to all three assemblies, and 20.51% of the sequencing reads make up a shared pool of single sequencing reads (singletons).
Here, we have analyzed the protein-protein interactions between the seven assembly factors bound to late cytoplasmic pre-40S ribosomes using recombinant proteins in binding assays.
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between the two conventions
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