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We found a good agreement between the two alignment methods in the seven experiments where both blood vessels and electroporated neurons were used.
Most of the length variation was concentrated in the hypervariable regions of the LSU fragment, while for the rrnL and SSU genes the length variation between the two alignment methods used (MF and PR) was relatively small (Table 2).
Restricting the analysis to SNPs with at least 10 counts per allele and genes with at least 2 measurements only marginally increases coincidence of genes with allelic RNA expression ratios ≥2 between the two alignment methods (69%).
Approximately 90% (depending on the applied FDR) of genes under positive selection based on the PRANK alignments are also detected when using MUSCLE alignments, suggesting that the majority of positively selected genes are consistent between the two alignment approaches.
All results were highly congurent between the two alignment methods; we therefore present parameter estimations derived from alignments using MUSCLE, and present only those derived from T-Coffee which would lead to different conclusions.
Despite the large differences in the length of the combined sequence between the two alignment methods (mostly due to LSU), the changes in the topology or level of support of the resulting trees were negligible (see below).
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Only 120 of the 33.6 million (0.0003 %) reads were aligned differently between the two alignments.
To emphasize, overlapping denotes some coverage of common sequence ranges between the two alignments for the same domain model.
When the distance between two alignments on the same chromosome was short, the genomic sequence was further analyzed by dot plot to identify any potential continuity between the two alignments.
The second is a Genomic Distance Penalty (GDP) calculated as log10 of the number of base pairs along the reference genome between the two alignments.
All invertebrate-type lysozymes could be reliably aligned to each other at both protein (alignment 2; Fig. 3) and DNA sequence level (alignment 3; position of indels is identical between the two alignments).
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between the two concordance
between the two requirements
between the two activities
between the two affected
between the two transition
between the two calibration
between the two aligned
between the two linear
between the two mapping
between the two correlations
between the two correlation
between the two coordinate
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