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Cell viability is effectively invariant for the various concentrations of Y2O3 spherical nanoparticles, with no statistically significant difference between the treated samples and the control.
We calculated the ratios from either the comparison between the treated samples against a common baseline (median expression of all control experiments) or by doing paired ratios between each treated sample and its corresponding control.
The difference between the treated samples and the untreated controls was analyzed by one way ANOVA followed by Tukey multiple comparison test in which p < 0.05 was considered as significant.
In total, 12 Affymetrix Genechips measurements were obtained with total RNA samples from which 6 duplicated comparisons between the treated samples and the respective control were analysed: 2 for the DTT treatment, 2 for the tunicamycin treatment and 2 for the t-PA production.
Mean percentage of hemolysis seemed to decrease in the case of treated samples (1.487% and 0.587% for 30 min and 60 min HCl-treated samples) compared with the untreated (9.123%) mPE, insinuating lesser damage and interaction between the treated samples and RBC.
The LSD- t test of one-way analysis of variance was performed to determine the significant differences in expression levels between the treated samples at 1, 2, 4, and 8 h and those at 0 h using the SPSS software package (version 13.0).
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The course of the effect differed in-between the treated samples, and only for pH 6 to pH 7 all samples remained within the 15% limit.
The language and statistical environment R was employed in order to explore the relationship between the control and the treated samples.
There were no differences between the controls and the treated samples in regards to the cellular structure and tissue integrity as observed by light microscopy.
The differences in tumour growth were not a product of alterations in cell proliferation as there was no change in the median number of proliferating cells in the cell lines between the control and the treated samples as assessed by Ki67 (Table 1).
A broad peak is also shown between 18° and 19° for the treated samples.
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