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Isozyme exchange was observed between the transcriptome data sets, with selective upregulation of multi-copy genes involved in cell division, fatty acid synthesis, solute binding, oxidative stress response and transcriptional regulation.
The results of pair-by-pair comparisons between the transcriptome data and qRT-PCR analysis and qRT-PCR primers are shown in Additional file 14: Table S8.
We therefore assessed whether a transcriptional association between pfcrt and pfmdr1 might account for the similarities we observed between the transcriptome data sets.
We tested for the genotypic effect of strain with an ANOVA and for correlations between the transcriptome data and our estimates of gene-specific CGV using the Spearman Rank method.
In addition, Western blots have shown a good correspondence between the transcriptome data and the proteome data since Pma1p was detected in high amounts only in the germinated conidia (see Additional file 12).
In this paper, in order to reveal the genetic factors underlying the divergence between the grain and pea aphids, comparative analysis between the transcriptome data of grain aphid generated in our lab by Roche 454 GS-FLX pyrosequencing and mRNA sequences of pea aphid available in the public databases was performed.
Similar(54)
We sought to determine the similarities between our qRT-PCR results and the transcriptome data published by MPGR.
Overall, as observed for the transcriptome data sets, the similarity between the two kinds of calculated probabilities (measured by the MSE) was greatest when probabilities were calculated using a mononucleotide composition model, and decreased when dinucleotide and trinucleotide models were used.
Small sample size in the pooled transcriptome libraries likely contributed to the observed inconsistencies between HRM genotypes and the allele frequencies predicted from the transcriptome data.
Moreover, the transcriptome data shows little genetic variation between laboratory Yukon Eutrema plants and those found at a field site.
To validate the transcriptome data we first compared genes differentially regulated between K56-2 and the cepR mutant to data from previous studies on CepRI regulation in this strain.
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