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Furthermore, there is a distinct difference between the total expression of the different mitochondrial genes.
Considering expression patterns for duplicated genes in a polyploid, relative to their orthologs in related diploids, twelve possible categories have been described, where each category corresponds to a combination of relative equalities or inequalities between two diploids and between the total expression in the tetraploid [ 18, 19, 27].
To deconvolute gene expression measured from samples composed of several cell types, PSEA relies on the detection of a correlation between the total expression level of a gene of interest and the expression levels of marker genes, i.e. genes expressed in a single cell type only.
Similar(57)
Therefore, it can be concluded that the total expression of orthologs between Z. marina and P. oceanica is significantly similar.
There was also a negative correlation between total expression level of the gene and ω (rs = -0.071, df = 10,711, p < 0.0001, Additional file 1: Appendix s6).
56 It should be cautioned that a major caveat associated with RNAi screening is potential off-target effects and the potential disconnect between reduction of total expression and inhibition of kinase function.
Expression levels are depicted as the ratio between the total L1-CAM expression level and β-actin levels in each brain area (i.e. L1-CAM/β-actin), normalized to the Naïve group.
We thus asked if these genes were expressed by cell types not taken into account in our deconvolution (possibly causing spurious association with the oliogdendrocyte reference signal) and tested for association between total expression and marker genes of additional minor cell types (see Methods).
Furthermore, we found a correlation between the total TCF12 protein expression level and progression free survival.
This linear relationship between total expression and population size in collections of heterogeneous tissue samples has been verified in previous studies [ 29].
Of the 7712 genes with moderate expression of at least 5 reads per million (RPM), 1796 genes showed significant correlation between total expression and somite number (q < 0.05, Spearman correlation).
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