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The analysis revealed major differences in the abundance of many cell types not just between the tissue types, but also between healthy, NAT and tumors (Supplementary Figs. 22 and 23).
This indicates significant differences in gene expression patterns between the tissue types.
Only the differentiation between the tissue types nerve/fat was enhanced under laser ablation conditions.
Of particular note were the relationships between the tissue types (which had been independently derived from the tissue model) and several key chemical components.
In each of these cases, there was "blending" or "flow" between the tissue types, without any sharp interfaces or clear demarcation to distinguish them.
The posterior probabilities were used by kmeans clustering in R to create a heatmap of methylation relationships between the tissue types.
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The results show that in the top 20 comparisons, very few genes overlapped between any pair of the tissue types, except for central nervous system (CNS) versus brain.
Figure 2a shows a plot of the various sources of variance associated with ANOVA of the data and it is notable that the major source of variance is attributable to the differences between the two tissue types and the variance associated with the scan date is negligible and very close to the type 1 error.
To learn more about the types of gene expression differences between the two tissue types, the 496 differentially expressed genes (listed in additional files 1 and 2) were placed into functional categories using Gene Ontology (GO) Annotations.
We also note the existence of TLR2 and TLR4 in both Blood_PBMC and Synovial Fibroblast as significant as this overlap may lead us to conclude that the interaction between the two tissue types is mediated by the TLR signaling pathway.
The differences observed in the LMVD between the three tissue types were statistically significant (Friedman test, P < 0.01).
More suggestions(15)
between the tissue specimens
between the tissue cells
between the tissue groups
between the tissue layers
between the tissue components
between the tissue preparations
between the tissue microenvironments
between the substrate types
between the cohort types
between the tissue levels
between the tissue samples
between the provider types
between the house types
between the tissue fractions
between the lattice types
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